Document Type : Original Research Article

Authors

Department of Chemistry, Yadegar-e-Imam Khomeini (RAH) Branch, Islamic Azad University, Tehran Iran

Abstract

A novel, rapid, simple and sensitive dispersive liquid-liquid microextraction method based on the solidification of floating organic drop (DLLME-SFO) combined with high performance liquid chromatography-ultra violet detection (HPLC-UV) was used to determine acrylamide in potato chips. The derivation of the acrylamide happened in the presence of KBr, KBrO3, H2SO4. Based on studies, 1-undecanol was selected as the extraction solvent. The factors affecting the extraction efficiency of DLLME-SFO such as the volume of the extraction solvent, kind and volume of the disperser solvent, effect of concentration of KBr, KBrO3, H2SO4 and time of centrifuge, time of derivative and extraction time were investigated and the optimal extraction conditions were estimated. Under the optimum conditions (extraction solvent: 150µl 1-undecanol;disperser solvent:1000 µl acetone; concentration of KBr, KBrO3:10 ppb; concentration of H2SO4 0.001 mol/l; extraction time: 3min), calibration curve is linear in the range of 0.5-15 ppb and correlation of determination (R2) is 0.993.The method was successfully applied for the determination of acrylamide in the actual potato chips.

Graphical Abstract

Preconcentration of acrylamide with dispersive liquid-liquid microextraction based on solidification of floating organic drop prior to determination by HPLC

Keywords

Main Subjects

[1] Y. Yamini, M. Ghanbarian, A. Esrafili, N. Yazdanfar, M. Moradi, Intern.J. Environ. Anal. Chem., 2012, 92, 1493-1505.
[2] P.D. DeArmond, A.L. DiGorgorio, Anal. Bioanal. Chem., 2013, 405, 4159-4166.
[3] R. Krska,A. Becaleski, E. Braekevelt, T. Koerner , X. Cao, R. Dabeka, S. Godefroy, B. Lau, J. Moisey, D.F.K. Rawn, P.M. Scott, Z. Wang, D. Forsyth,  Anal. Bioanal. Chem., 2012, 402,139-162.
[4] M. Franek, D. Rubio, I. Diblikova, F. Rubio, Talanta, 2014, 123, 146-150.
[5] V.A. Yaylayan, R.H. Studler, J. AOAC Int., 2005, 88,262-267.
[6] J. Liu, G. Zhao, Y. Yuan, F. Chen, X. Hu, Food chemistry, 2008, 108, 760-767.
[7] L. Jiang, J. Cao, Y. An, C. Geng, S. Qu, L. Jiang, L. Zhong, Toxicol. In Vitro, 2007, 21, 1486-1492.
[8] IARC. IARC Monographs on the Evaluation of Carcinogenic Risks to Humans, IARC: Lyon, France, 1994, 389-391.
[9] FAO/WHO Consultations on the Health Implications of Acrylamide in Food, Report of a Joint FAO/WHO Consultation, Geneva, Switzerland,June, 2002, 25-27.
[10] JIFSAN/NCFST, 2002, Acrylamide in food workshop: scientific issues, uncertainties, and research strategies, Workshop on 28–30 October 2002, Rosemont,IL,USA. http://www.jifsan.umd.edu/acrylamide2002.htm.
[11] J.S .Elmore, A. Briddon, A.T. Dodson, Nira Muttucumaru, N.G. Halford, D.S. Mottram, Food chemistry, 2015, 182,1-8
[12] D.S Mottram, B.L. Wedzicha, A.T. Dodson, Nature, 2002, 419, 448-449.
[13] E. Psillakis, N. Kalogerakis, TrendsAnal. Chem., 2002, 21, 54-64.
[14] H. Prosen, H. Zupancic-Kralj, Trends Anal. Chem., 1999, 18, 272-282.
[15] M. Rezaee, Y. Assadi, M. R. Milani Hosseini, E. Aghaee, F. Ahmadi, S. Berijani, J. Chromatogr. A, 2006, 1116, 1-9.
[16] M.I. Leong, S.D. Huang, J. Chromatogr. A, 2008, 1211, 8-12.
[17] M.R. Khalilizanjani, Y. Yamini, S. Shariati, J.A. Jonsson, Anal. Chim. Acta., 2007, 585, 286-291.
[18] L. Lili, H. Xu, D.S. Yanfang Cui, S. Hu, G. Zhang, J. Chromatogr. A, 2010, 1217, 2365-2370.
[19] M.M. Sangai, H.H. Abbas, W.A. Wan Ibrahim, H.Y. Aboul-Enien, Food Chemistry, 2012, 133, 557-562.
[20] C. Zheng, J. Zhao, P. Bao, J. Gao, J. He, Chromatogr. A., 2011, 1218, 3830-3836.
[21] M. Mirzaei, M., Behzadi, N. Mahmoud Abadi, A. Beizaei, J. Hazard. Mater., 2011, 186, 1739-1743.
[22] J.H. Suh, Y.Y. Lee, H.J. Lee, M. Kang, Y. Hur, S.N. Lee, D.H. Yang, S. Beom Han, J. Pharma.Biomed. Anal., 2013, 75, 214-219.
[23] T. Ahmadi-Jouibari, N. Fattahi, M. Shamsipur, M. Pirsaheb, J. Pharma. Biomed. Anal., 2013, 85, 14-20.
[24] I. Kohlera, J. Schappler, T. Sierro, S. Rudaz, J. Pharma. Biomed. Anal., 2013, 73, 82-89.
[25] A. Becalski, B.P.Y. Lau, D. Lewis, S.W. Seaman,J.Agric. Food Chem., 2003, 51, 802-809.
 
[26] T. Wenzl, M.B. Delaculle, E. Anklam, Food ADDIT. Contam., 2003, 20, 885-891.
[27] A. Pittet, A. Perisset, J.M. Oberson, J. Chromatogr.A, 2004, 1035, 123-129.
[28] E. Tareke, P. Rydberg, P. Karlsson, S. Eriksson, M. Törnqvist, Chem. Res. Toxicol, 2000, 13, 517-524.
[29] H. Ono, Y. Chuda, M. Ohnishi-Kameyama, H. Yada , M. Ishizaka , H. Kobayashi , M. Yoshida, Food Addit. Contam., 2003, 20, 215-222.
 
[30] J.S. Ahn, L. Castle, D.B. Clarke, A.S. Lloyd , M.R. Philo , D.R. Speck,  Food Addit. Contam., 2002, 19, 1116-1121.
[31] Y. Zhang, Y. Dong, Y. Ren, Y. Zhang, J. Chromatogr.A, 2006, 1116, 209-216.
[32] M. Lee, L. Chang, J. Dou, Anal. Chim. Acta, 2007, 582, 19-23.
[33] K. Granby, S. Fagt, Anal. Chim. Acta, 2004, 520, 177-182.
[34] S. Cavalli, S. Polesello, G. Saccani, J. Chromatogr.A, 2004, 1039, 155-159.